瓊脂糖結(jié)合番茄凝集素 免疫細(xì)胞組織
簡(jiǎn)要描述：
瓊脂糖結(jié)合番茄凝集素 免疫細(xì)胞組織 番茄凝集素（Tomato lectin, Lycopersicon Esculentum lectin (LEL)）,是一種非常穩(wěn)定的單亞基糖蛋白，約含有等量的蛋白和碳水化合物。
產(chǎn)品時(shí)間：2024-05-29
打印當(dāng)前頁(yè)Agarose bound Lycopersicon Esculentum (Tomato) Lectin (LEL, TL)
AL-1173瓊脂糖結(jié)合番茄凝集素
搜索關(guān)鍵詞：
番茄凝集素；血管內(nèi)灌注；凝集素灌注；微血管；內(nèi)皮細(xì)胞；LEA；TL-1176 ; VECTOR; LEL,TL; LAB
產(chǎn)品訂購(gòu)：【VECTOR】原裝好貨，現(xiàn)貨供應(yīng)
品牌 | 產(chǎn)品名稱(chēng) | 產(chǎn)品編號(hào) | 規(guī)格 | 價(jià)格 |
VECTOR | Agarose bound Lycopersicon Esculentum (Tomato) Lectin (LEL, TL)
| AL-1173 | 2mg | 3800 |
【溫馨提示：見(jiàn)我司懋康生物整理的血管功能研究標(biāo)志物-番茄凝集素產(chǎn)品專(zhuān)題】
產(chǎn)品描述：
番茄凝集素（Tomato lectin, Lycopersicon Esculentum lectin (LEL)）,是一種非常穩(wěn)定的單亞基糖蛋白，約含有等量的蛋白和碳水化合物。據(jù)報(bào)道其糖類(lèi)部分由85%半乳糖組成，在溶液中可能形成多聚體聚合物。番茄凝集素雖然和土豆凝集素、曼陀羅凝集素（DSL）和麥胚凝集素（WGA）有部分相同的特異性、但在許多多面呈現(xiàn)出du特之處。番茄凝集素LEL能夠很好地與血型糖蛋白（glycophorin）和tamm-Horsfall糖蛋白結(jié)合，能夠有效結(jié)合和標(biāo)記嚙齒類(lèi)動(dòng)物的血管內(nèi)皮（vascularendothelium）
保存與運(yùn)輸方法：
保存：2-8℃干燥保存、有效期一年
運(yùn)輸：冰袋運(yùn)輸。
Overview
Features:
Matrix is heat stable, cross-linked 4% agarose beads with a molecular exlusion of about 2x107 daltons
Bead diameter ranges in size from 45-165 microns
Matrix is stable in solutions at pH 3-11 as well as many organic solvents
Immobilized lectins are prepared using affinity purified lectins
Covalent attachment preserves lectin activity and minimizes conformational changes that might result in nonspecific or hydrophobic interactions
Hydrophilic spacer arm is inserted between the lectin and the matrix
Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
No residual charges present after conjugation. This minimizes non-specific binding to the matrix
Product supplied as a 1:1 suspension in buffer
2 mg lectin/ml gel
Inhibiting/Eluting Sugar: Chitin Hydrolysate or Glycoprotein Eluting Solution (ES-5100)
Cat. No. | AL-1173-2 |
Unit Size | 2 ml |
Country of Manufacture | United States |
Applications | Glycobiology, Affinity Chromatography |
Matrix Conjugate | Lectins |
Sugar Specificity | [GlcNAc]1-3, N-Acetylglucosamine |
Conjugate | Agarose |
More Information
Tomato lectin is a very stable single subunit glycoprotein containing about 50 percent arabinose and galactose and may form multimeric aggregates in solution. Tomato lectin, although sharing some specificities with potato lectin, Datura lectin, and wheat germ agglutinin, has been reported to be dissimilar in many respects. LEL binds well to glycophorin and Tamm-Horsfall glycoprotein and has been used effectively to label vascular endothelium in rodents.
Agarose bound* tomato lectin is prepared using our affinity-purified lectins. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2x107 daltons are used as the solid-phase matrix to which the lectins are covalently coupled. The attachment of the lectins to the beads is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins that might result in nonspecific ionic or hydrophobic interactions. The technique we have developed to couple lectins to agarose beads inserts a hydrophilic spacer arm between the lectin and the matrix.
This coupling method provides several advantages over the traditional cyanogen bromide procedure:
Maximum carbohydrate binding activity of the coupled lectins is retained
Linkage is stable over a range of pH values
Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
No residual charges are present after conjugation. This minimizes non-specific binding to the matrix.
Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins.
Inhibiting/Eluting Sugar: Chitin Hydrolysate
*2 mg lectin/ml gel
注意事項(xiàng)：
為了您的安全和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。
另我司懋康生物提供自主品牌MKbio的番茄凝集素產(chǎn)品，質(zhì)量保證，現(xiàn)貨供應(yīng)
產(chǎn)品名稱(chēng) | 產(chǎn)品編號(hào) | 規(guī)格 | 目錄價(jià) |
FITC labeled Lycopersicon Esculentum(Tomato)Lectin(Ex/Em=495/515nm,綠色熒光） | MP6311-500UG | 500ug | 1400 |
— —Written/Edited by V. Shallan【版權(quán)歸MKBio懋康所有】
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瓊脂糖結(jié)合番茄凝集素 免疫細(xì)胞組織瓊脂糖結(jié)合番茄凝集素 免疫細(xì)胞組織